Autophagy induced by infectious pancreatic necrosis virus promotes its multiplication in the Chinook salmon embryo cell line CHSE-214.
Identifieur interne : 000146 ( Main/Exploration ); précédent : 000145; suivant : 000147Autophagy induced by infectious pancreatic necrosis virus promotes its multiplication in the Chinook salmon embryo cell line CHSE-214.
Auteurs : Ying Dong [République populaire de Chine] ; Jingzhuang Zhao [République populaire de Chine] ; Xiaoyu Chen [République populaire de Chine] ; Miao Liu [République populaire de Chine] ; Guangming Ren [République populaire de Chine] ; Tongyan Lu [République populaire de Chine] ; Yizhi Shao [République populaire de Chine] ; Liming Xu [République populaire de Chine]Source :
- Fish & shellfish immunology [ 1095-9947 ] ; 2020.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Autophagie (MeSH), Autophagosomes (ultrastructure), Autophagosomes (virologie), Embryon non mammalien (virologie), Infections à Birnaviridae (médecine vétérinaire), Infections à Birnaviridae (virologie), Lignée cellulaire (MeSH), Maladies des poissons (virologie), Microscopie électronique à transmission (médecine vétérinaire), Réplication virale (MeSH), Saumon (embryologie), Virus de la nécrose pancréatique infectieuse (physiologie).
- MESH :
- embryologie : Saumon.
- médecine vétérinaire : Infections à Birnaviridae, Microscopie électronique à transmission.
- physiologie : Virus de la nécrose pancréatique infectieuse.
- virologie : Autophagosomes, Embryon non mammalien, Infections à Birnaviridae, Maladies des poissons.
- ultrastructure : Animaux, Autophagie, Autophagosomes, Lignée cellulaire, Réplication virale.
English descriptors
- KwdEn :
- Animals (MeSH), Autophagosomes (ultrastructure), Autophagosomes (virology), Autophagy (MeSH), Birnaviridae Infections (veterinary), Birnaviridae Infections (virology), Cell Line (MeSH), Embryo, Nonmammalian (virology), Fish Diseases (virology), Infectious pancreatic necrosis virus (physiology), Microscopy, Electron, Transmission (veterinary), Salmon (embryology), Virus Replication (MeSH).
- MESH :
- embryology : Salmon.
- physiology : Infectious pancreatic necrosis virus.
- ultrastructure : Autophagosomes.
- veterinary : Birnaviridae Infections, Microscopy, Electron, Transmission.
- virology : Autophagosomes, Birnaviridae Infections, Embryo, Nonmammalian, Fish Diseases.
- Animals, Autophagy, Cell Line, Virus Replication.
Abstract
Infectious pancreatic necrosis virus (IPNV) is a common pathogen that causes huge economic losses for the salmonid aquaculture industry. Autophagy plays an important regulatory role in the invasion of pathogenic microorganisms. In this study, we explored the relationship between IPNV infection and autophagy in Chinook salmon embryo (CHSE-214) cells using standard methods. Transmission electron microscopy showed that IPNV infection produced typical structures of autophagosomes in CHSE-214 cells. Transformation of microtubule-associated protein 1 light chain 3 (LC3)-I to LC3-II protein, a marker of autophagy, was observed in IPNV-infected cells using confocal fluorescence microscopy and western blot analysis. Western blotting also showed that expression of the autophagy substrate p62 was significantly decreased in IPNV-infected cells. The influence of autophagy on IPNV multiplication was further clarified with cell culture experiments using autophagy inducer rapamycin and autophagy inhibitor 3-methyladenine. Rapamycin promoted IPNV multiplication at both the nucleic acid and protein levels, which led to higher IPNV yields; 3-methyladenine treatment had the opposite effect. This study has demonstrated that IPNV can induce autophagy, and that autophagy promotes the multiplication of IPNV in CHSE-214 cells.
DOI: 10.1016/j.fsi.2019.12.067
PubMed: 31874298
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Electronic address: lutongyan@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070</wicri:regionArea><wicri:noRegion>150070</wicri:noRegion></affiliation></author><author><name sortKey="Shao, Yizhi" sort="Shao, Yizhi" uniqKey="Shao Y" first="Yizhi" last="Shao">Yizhi Shao</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. 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Electronic address: xuliming@hrfri.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070</wicri:regionArea><wicri:noRegion>150070</wicri:noRegion></affiliation></author></analytic><series><title level="j">Fish & shellfish immunology</title><idno type="eISSN">1095-9947</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Autophagosomes (ultrastructure)</term><term>Autophagosomes (virology)</term><term>Autophagy (MeSH)</term><term>Birnaviridae Infections (veterinary)</term><term>Birnaviridae Infections (virology)</term><term>Cell Line (MeSH)</term><term>Embryo, Nonmammalian (virology)</term><term>Fish Diseases (virology)</term><term>Infectious pancreatic necrosis virus (physiology)</term><term>Microscopy, Electron, Transmission (veterinary)</term><term>Salmon (embryology)</term><term>Virus Replication (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Autophagie (MeSH)</term><term>Autophagosomes (ultrastructure)</term><term>Autophagosomes (virologie)</term><term>Embryon non mammalien (virologie)</term><term>Infections à Birnaviridae (médecine vétérinaire)</term><term>Infections à Birnaviridae (virologie)</term><term>Lignée cellulaire (MeSH)</term><term>Maladies des poissons (virologie)</term><term>Microscopie électronique à transmission (médecine vétérinaire)</term><term>Réplication virale (MeSH)</term><term>Saumon (embryologie)</term><term>Virus de la nécrose pancréatique infectieuse (physiologie)</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Saumon</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Salmon</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Birnaviridae</term><term>Microscopie électronique à transmission</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la nécrose pancréatique infectieuse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Infectious pancreatic necrosis virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Autophagosomes</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Birnaviridae Infections</term><term>Microscopy, Electron, Transmission</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Autophagosomes</term><term>Embryon non mammalien</term><term>Infections à Birnaviridae</term><term>Maladies des poissons</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Autophagosomes</term><term>Birnaviridae Infections</term><term>Embryo, Nonmammalian</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Autophagy</term><term>Cell Line</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Autophagosomes</term><term>Lignée cellulaire</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infectious pancreatic necrosis virus (IPNV) is a common pathogen that causes huge economic losses for the salmonid aquaculture industry. Autophagy plays an important regulatory role in the invasion of pathogenic microorganisms. In this study, we explored the relationship between IPNV infection and autophagy in Chinook salmon embryo (CHSE-214) cells using standard methods. Transmission electron microscopy showed that IPNV infection produced typical structures of autophagosomes in CHSE-214 cells. Transformation of microtubule-associated protein 1 light chain 3 (LC3)-I to LC3-II protein, a marker of autophagy, was observed in IPNV-infected cells using confocal fluorescence microscopy and western blot analysis. Western blotting also showed that expression of the autophagy substrate p62 was significantly decreased in IPNV-infected cells. The influence of autophagy on IPNV multiplication was further clarified with cell culture experiments using autophagy inducer rapamycin and autophagy inhibitor 3-methyladenine. Rapamycin promoted IPNV multiplication at both the nucleic acid and protein levels, which led to higher IPNV yields; 3-methyladenine treatment had the opposite effect. This study has demonstrated that IPNV can induce autophagy, and that autophagy promotes the multiplication of IPNV in CHSE-214 cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31874298</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9947</ISSN><JournalIssue CitedMedium="Internet"><Volume>97</Volume><PubDate><Year>2020</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Fish & shellfish immunology</Title><ISOAbbreviation>Fish Shellfish Immunol</ISOAbbreviation></Journal><ArticleTitle>Autophagy induced by infectious pancreatic necrosis virus promotes its multiplication in the Chinook salmon embryo cell line CHSE-214.</ArticleTitle><Pagination><MedlinePgn>375-381</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1050-4648(19)31196-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fsi.2019.12.067</ELocationID><Abstract><AbstractText>Infectious pancreatic necrosis virus (IPNV) is a common pathogen that causes huge economic losses for the salmonid aquaculture industry. Autophagy plays an important regulatory role in the invasion of pathogenic microorganisms. In this study, we explored the relationship between IPNV infection and autophagy in Chinook salmon embryo (CHSE-214) cells using standard methods. Transmission electron microscopy showed that IPNV infection produced typical structures of autophagosomes in CHSE-214 cells. Transformation of microtubule-associated protein 1 light chain 3 (LC3)-I to LC3-II protein, a marker of autophagy, was observed in IPNV-infected cells using confocal fluorescence microscopy and western blot analysis. Western blotting also showed that expression of the autophagy substrate p62 was significantly decreased in IPNV-infected cells. The influence of autophagy on IPNV multiplication was further clarified with cell culture experiments using autophagy inducer rapamycin and autophagy inhibitor 3-methyladenine. Rapamycin promoted IPNV multiplication at both the nucleic acid and protein levels, which led to higher IPNV yields; 3-methyladenine treatment had the opposite effect. This study has demonstrated that IPNV can induce autophagy, and that autophagy promotes the multiplication of IPNV in CHSE-214 cells.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China; National Demonstration Center for Experimental Fisheries Sciences Education, Shanghai Ocean University, Shanghai, 201306, PR China. Electronic address: 1909662504@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Jingzhuang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. Electronic address: zhaojingzhuang@hrfri.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xiaoyu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Technology Center of Wuhan Customs, Wuhan, 430050, PR China. Electronic address: xychen@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Miao</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. Electronic address: 446048847@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Guangming</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. Electronic address: renguangming@hrfri.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Tongyan</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. Electronic address: lutongyan@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shao</LastName><ForeName>Yizhi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. Electronic address: shaoyizhi@hrfri.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Liming</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, PR China. Electronic address: xuliming@hrfri.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>12</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Fish Shellfish Immunol</MedlineTA><NlmUniqueID>9505220</NlmUniqueID><ISSNLinking>1050-4648</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000071182" MajorTopicYN="N">Autophagosomes</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="Y">Autophagy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018175" MajorTopicYN="N">Birnaviridae Infections</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004625" MajorTopicYN="N">Embryo, Nonmammalian</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005393" MajorTopicYN="N">Fish Diseases</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018061" MajorTopicYN="N">Infectious pancreatic necrosis virus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012474" MajorTopicYN="Y">Salmon</DescriptorName><QualifierName UI="Q000196" MajorTopicYN="N">embryology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="Y">Virus Replication</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Autophagy</Keyword><Keyword MajorTopicYN="N">Chinook salmon embryo cells (CHSE-214)</Keyword><Keyword MajorTopicYN="N">Infectious pancreatic necrosis virus (IPNV)</Keyword><Keyword MajorTopicYN="N">LC3</Keyword><Keyword MajorTopicYN="N">p62</Keyword></KeywordList><CoiStatement>Declaration of competing interest All authors declare that no conflicts of interest exist.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>12</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31874298</ArticleId><ArticleId IdType="pii">S1050-4648(19)31196-9</ArticleId><ArticleId IdType="doi">10.1016/j.fsi.2019.12.067</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Dong, Ying" sort="Dong, Ying" uniqKey="Dong Y" first="Ying" last="Dong">Ying Dong</name></noRegion><name sortKey="Chen, Xiaoyu" sort="Chen, Xiaoyu" uniqKey="Chen X" first="Xiaoyu" last="Chen">Xiaoyu Chen</name><name sortKey="Liu, Miao" sort="Liu, Miao" uniqKey="Liu M" first="Miao" last="Liu">Miao Liu</name><name sortKey="Lu, Tongyan" sort="Lu, Tongyan" uniqKey="Lu T" first="Tongyan" last="Lu">Tongyan Lu</name><name sortKey="Ren, Guangming" sort="Ren, Guangming" uniqKey="Ren G" first="Guangming" last="Ren">Guangming Ren</name><name sortKey="Shao, Yizhi" sort="Shao, Yizhi" uniqKey="Shao Y" first="Yizhi" last="Shao">Yizhi Shao</name><name sortKey="Xu, Liming" sort="Xu, Liming" uniqKey="Xu L" first="Liming" last="Xu">Liming Xu</name><name sortKey="Zhao, Jingzhuang" sort="Zhao, Jingzhuang" uniqKey="Zhao J" first="Jingzhuang" last="Zhao">Jingzhuang Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Autophagy induced by infectious pancreatic necrosis virus promotes its multiplication in the Chinook salmon embryo cell line CHSE-214.
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